A Secret Weapon For flow cytometers
A Secret Weapon For flow cytometers
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Information-enriched characterization of mobile populations. Morphological facts from photographs can increase to your richness of flow cytometry facts. For instance, the determine shows an normally typical apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Every single inhabitants to reveal morphologically unique options. These insights couldn't happen to be gained from multiplex staining by itself.
As samples are obtained on the Attune CytPix Flow Cytometer, the large-pace brightfield digicam captures and outlets visuals of detected occasions at a charge around 6,000 photographs per 2nd, depending upon the flow level and image size. For better overall flexibility, Attune Cytometric Software program allows you to alter the picture capture frequency as wanted and choose a specific gate for graphic seize, if demanded.
Combine acoustic concentrating know-how with significant-parameter Assessment for versatile spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a significant-velocity brightfield camera for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.
"An image is not quite really worth a thousand dots, but it genuinely helps you to see pictures. It is great to discover images that correlate with staining. By way of example, AnnexinV-favourable cells which are morphologically various than healthy cells, or CD14+ monocytes which are much larger plus more textured than CD3+/CD4+/CD14- lymphocytes.
In addition, it is actually illuminating to determine the amount of doublets slip by conventional singlet gates, and excellent to validate the position of surprising double-favourable events in my analysis."
Optimizing gating technique. Even strong handbook singlet gating is error-vulnerable and continues to be a subjective choice stage in Just about all flow cytometry assays. Imaging can be used to confirm and modify gates to include only solitary cells of fascination.
Our scientists will design panels to suit your needs as repeatedly you need—cost-free, no merchandise acquire desired.
The Attune NxT cytometer is definitely the dependable workhorse instrument your lab can trust in for reliable and reliable effectiveness. The Attune CytPix design includes a higher-speed brightfield digital camera and AI-driven picture Assessment computer software, with new updates such as device Discovering tools and an intuitive person interface, enabling Increased data precision, precision, and more quickly insights into sample biology.
Users can perspective event photos in serious-time to verify sample good quality, enhance protocols, and history the morphology of each cell population recognized for Examination. Imaging seize also Rewards from acoustic concentrating, which really helps to placement the cells to ensure that a pointy, centered impression is received.
Seem waves enable precise cell alignment, causing Outstanding details high-quality and ~10X speedier sample flow charges.
Software note: Automated impression Examination lessens consumer-to-person variability in flow cytometry gating techniques
Leverage the strength of Superior technological innovation and innovation to accelerate your discoveries. Rely on while in the Attune Flow Cytometer family to check here provide the trustworthiness, pace, and precision that set your work apart.
No wash/no lyse leukocyte analysis with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in one mM EDTA ( Qualified UX/UI permits consumers to teach AI styles for expanded purposes. (A) The “Practice Product” function gives annotation instruments and authentic-time design instruction accuracy.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technology, which makes use of sound waves to meticulously align cells in the flow mobile. This innovative method surpasses traditional hydrodynamic methods, resulting in more rapidly processing speeds, Remarkable knowledge quality, and greater sample throughput.
The Attune Xenith cytometer is often a higher-parameter spectral analyzer, offering equally spectral unmixing and standard flow capabilities for comprehensive and flexible cell Examination.
Agilent Worth Assure ensures you at the least a decade of instrument use from the day you purchase. Usually, we will credit history you Together with the residual worth of the process toward an upgraded product.
What appeared to be a homogeneous inhabitants is definitely extra diverse—an Perception that ought to be viewed as when interpreting outcomes.